Fig 2: Anti-CB2R antibodies. The letters assigned to the antibodies correspond to those assigned in Table 1. (A) Recognition of CB2R by antibodies used on nervous tissue. Antibodies directed against the N-terminal (N-term) region of the CB2R receptor are shown in orange. Antibodies against the C-terminal (C-term) region of the receptor are shown in pink. (B) CB2R antibodies tested in CB2R partial Knock-Out mice. The antibodies which have been tested on tissue from each of the two available partial KO mouse are represented, that is on the Zimmer strain 28 in which CB2R C-term region is deleted and on the Deltagen strain (The Jackson Laboratory, Cnr2 tm1Dgen /J, #005786) in which CB2R N-term region is deleted. The antibodies tested on tissue from “non-adequate” partial KO mice, that is to say in which the deleted part of the CB2R gene does not encode the epitope recognized by the antibody, are represented in gray. Conversely, the antibodies which have been tested on “adequate” partial KO mouse tissue are represented in colors, that is, in orange and pink for those recognizing the N-term and C-term regions, respectively. The antibodies validated by these tests, that is to say for which a reduction in signal was observed on the tissue of the appropriate KO mouse, are represented in green. C-term, C-terminal; CB2R, cannabinoid receptor type 2; KO, knock-out; N-term, N-terminal. Figure generated with https://biorender.com/